Membrane Filters - Classification and Recommendation
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Selecting Right Membrane Filter
- Clearly define your application requirements
- Choose a compatible membrane
- Select the most effective and efficient pore size
Selecting Membranes for Your Applications
- Determine the particle size to be retained or filtered. Membrane will retain all particles equal to and larger than their designated pore size.
- Assess the chemical compatibility of the membrane filter with the liquid or gas to be filtered. Consider the chemical resistance properties of all parts that will contact the filter.
- Consider hydrophilic or hydrophobic membrane; temperature, flow rate, throughput, and sterilization needs.
Membrane filter materials and properties
|
Filter Media |
Nylon/PTFE/PTFE L/PES/PVDF/MCE/CA/GF/PP/Active Carbon |
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|
Pore Size, μm |
0.1μm, 0.22μm, 0.45μm, 0.65μm, 0.8μm, 1.0μm, 3.0μm, 5.0μm, 10μm |
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|
Pre-filter |
1um Glass Fiber or PP |
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|
Diameter |
4mm |
13mm |
25mm |
33mm |
50mm |
|
Housing |
PP |
PP |
PP |
PP |
PP |
|
Filtration Area |
0.2cm2 |
1.3cm2 |
4.9 cm2 |
8.5 cm2 |
19.8cm2 |
|
Maximum Operating Pressure |
5.2bar |
5.2bar |
5.2bar |
5.2bar |
5.2bar |
|
Process Volume |
2ml |
10ml |
100ml |
200ml |
5000ml |
|
Hold-up Volume |
<10ul |
< 50ul |
<100ul |
<200ul |
<350ul |
|
Inlet |
Female |
Female |
Female |
Female |
Both inlet and outlet is 7~13 mm stepped hose barb connection with 6:100 Luer slip |
|
Outlet |
Male |
Male |
Male |
Male |
|
|
Flow Direction |
Flow should enter from the inlet |
Both is fine |
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|
Maximum Operating Temperature |
131℃ |
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|
Sterilization |
Autoclave at 121℃ at 1 bar for 20 minutes/EO/Gamma |
By EO or 3 autoclave cycles of 30 min at 123℃. |
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- Hydrophilic membrane. Resistant to a range of organic solvents. Suitable for use with high pH samples. Binds proteins, which makes it unsuitable for protein recovery applications.
Hydrophobic Polytetrafluoroethylene(PTFE)
- Hydrophobic membrane. Resistant to organic solvents as well as strong acids and bases. Low protein binding. Main applications are filtration of non-aqueous samples. Prior to filtering of aqueous samples, if needed, the membrane must be pre-wetted with a water-miscible organic solvent.
Hydrophilic Polytetrafluoroethylene (PTFE)
- Hydrophilic PTFE membranes are ideal for HPLC and mixtures of aqueous and organic solvents. Use with both aqueous and organic solvents, along with their mixtures.
Hydrophilic Polytetrafluoroethylene (PTFE L)
- Hydrophilic PTFE membranes that are unsupported membrane that ideal for HPLC and mixtures of aqueous and organic solvents. Use with both aqueous and organic solvents, along with their mixtures.
Polytetrafluoroethylene( PES )
- Hydrophilic membrane. Broad solvent compatibility. Suitable for filtration of aqueous and compatible organic solvents. Higher liquid flow than either PTFE or PVDF. Low in extractables. Low protein binding.
- Hydrophilic membrane. Limited solvent resistance. Very low protein binding capacity, which makes it an excellent choice for protein recovery applications.
Polyvinylidene difluoride (PVDF)
- Hydrophobic membrane. Fast flow rate and very low protein binding. Generally compatible with most common solvents. Ideal for protein recovery applications.
- Hydrophilic MCE membranes. Biologically inert mixture of Cellulose Acetate and Cellulose Nitrate membranes which have higher protein binding than CA for most proteins. High porosity provides high flow rate. Good use for aqueous based samples.
Glass microfiber/glass fiber (GMF/GF)
- Hydrophilic material. Excellent compatibility with organic solvents and strong acids (apart from hydrofluoric acid) and bases. Either used as a prefilter or as a final filter.
- Slightly hydrophobic membrane. Resistant to a wide range of organic solvents. Also good choice used as a pre-filter or as a final filter.
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