SPE Cartridge - Protocol

Too see our SPE Cartridge classification and recommendation, click here.

Operation Steps

According to the different sorbent retention mechanism (sorbent retain target analytes or retain impurities), the operation is slightly different.

1. Sorbent Retain Target Analytes

    SPE operations are generally four steps:

        (1) Conditioning and equilibration: Solvent is passed through the SPE material to wet the bonded functional groups => ensures consistent interaction.Sorbent/ phase is treated with a solution that is similar (in polarity, pH, etc.) to the sample matrix => maximizes retention.
        (2) Sample Load: Introduction of the sample = analytes of interest are bound/ extracted onto the phase/sorbent.
        (3) Washing: Selectively remove unwanted interference co-extracted with the analyte without prematurely eluting analytes of interest.
        (4) Elution: Removing analytes of interest with a solvent that overcomes the primary and secondary retention interactions between sorbent and analytes of interest.

2. Sorbent Retain Disruptors

    SPE operations are generally three steps:

        (1) Conditioning and equilibration： Solvent is passed through the SPE material to wet the bonded functional groups => ensures consistent interaction.Sorbent/ phase is treated with a solution that is similar (in polarity, pH, etc.) to the sample matrix => maximizes retention.
        (2) Sample Load: disruptors are bound/ extracted onto the phase/sorbent. Target analytes pass through cartridge with solution. Should collect solution in this step. 
        (3) Elution: Removing analytes of interest with a solvent that overcomes the primary and secondary retention interactions between sorbent and analytes of interest. Combine the solution with step (2).

 

Too see our SPE Cartridge classification and recommendation, click here.